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小動物活體成像的實驗及標記技術分類
小(xiao)動(dong)物(wu)(wu)(wu)活(huo)體成像(xiang)的(de)實驗主要分為(wei)兩(liang)大(da)類(lei)，一(yi)(yi)種(zhong)(zhong)是看腫瘤或(huo)者(zhe)炎癥的(de)發展以(yi)及腫瘤的(de)轉移。一(yi)(yi)種(zhong)(zhong)是看物(wu)(wu)(wu)質(zhi)在小(xiao)鼠(shu)體內的(de)代謝和(he)聚(ju)集情(qing)況。比如藥(yao)物(wu)(wu)(wu)代謝，基本上是要看不同的(de)時間(jian)內，注射進入(ru)小(xiao)鼠(shu)體內的(de)藥(yao)物(wu)(wu)(wu)到(dao)了小(xiao)鼠(shu)的(de)哪個(ge)部分的(de)器(qi)官以(yi)及量的(de)多少。那么(me)如果單純是細胞(bao)或(huo)者(zhe)是藥(yao)物(wu)(wu)(wu)，我們本身是無法看見(jian)的(de)。這就牽扯到(dao)，我們需要用到(dao)一(yi)(yi)些，可以(yi)被影(ying)像(xiang)設備捕捉到(dao)的(de)發光物(wu)(wu)(wu)質(zhi)，對(dui)細胞(bao)或(huo)者(zhe)藥(yao)物(wu)(wu)(wu)進行標記。從而指示細胞(bao)或(huo)者(zhe)藥(yao)物(wu)(wu)(wu)在小(xiao)鼠(shu)中存在的(de)位置(zhi)。小(xiao)動(dong)物(wu)(wu)(wu)活(huo)體標記技術主要分為(wei)兩(liang)種(zhong)(zhong)，一(yi)(yi)種(zhong)(zhong)是生物(wu)(wu)(wu)發光，一(yi)(yi)種(zhong)(zhong)是熒(ying)光。生物(wu)(wu)(wu)發光是通過熒(ying)光素酶(mei)（...
2022-04-27
植物活體成像都有哪些應用
在傳統(tong)的(de)(de)植(zhi)(zhi)物(wu)學(xue)(xue)研(yan)究中，受實驗(yan)手段所限只能研(yan)究植(zhi)(zhi)物(wu)表型在某一(yi)(yi)個時間(jian)截面時的(de)(de)靜止狀(zhuang)態，反映的(de)(de)是該時間(jian)點(dian)以前全部生(sheng)物(wu)學(xue)(xue)動態過(guo)程(cheng)的(de)(de)累(lei)積效應。然而(er)植(zhi)(zhi)物(wu)的(de)(de)生(sheng)長發(fa)(fa)育(yu)是一(yi)(yi)系列(lie)時間(jian)—空間(jian)特異的(de)(de)過(guo)程(cheng)的(de)(de)組(zu)合，只有觀(guan)察動態變化過(guo)程(cheng)才能更(geng)好地認識植(zhi)(zhi)物(wu)生(sheng)長發(fa)(fa)育(yu)調控(kong)的(de)(de)時空特異性。而(er)且隨著植(zhi)(zhi)物(wu)科(ke)學(xue)(xue)的(de)(de)發(fa)(fa)展，越來越多的(de)(de)研(yan)究人(ren)員需要在活體(ti)(ti)植(zhi)(zhi)株中驗(yan)證(zheng)基(ji)因(yin)的(de)(de)功(gong)能，研(yan)究基(ji)因(yin)的(de)(de)表達調控(kong)，以及(ji)(ji)植(zhi)(zhi)物(wu)的(de)(de)晝夜節律等(deng)，因(yin)此(ci)，對于植(zhi)(zhi)物(wu)活體(ti)(ti)成(cheng)像(xiang)的(de)(de)需求也越來越大，還可以應用于植(zhi)(zhi)物(wu)陽性克(ke)隆的(de)(de)篩(shai)選(xuan)以及(ji)(ji)轉基(ji)因(yin)植(zhi)(zhi)物(wu)的(de)(de)鑒定等(deng)多方面。植(zhi)(zhi)物(wu)活體(ti)(ti)成(cheng)像(xiang)可...
2022-03-09
介紹活體成像系統的技術原理
活(huo)體(ti)(ti)(ti)成像(xiang)(xiang)系(xi)統(tong)(tong)(tong)是(shi)一套非常靈敏(min)的光學(xue)檢測儀器(qi)，能夠(gou)直接監控活(huo)體(ti)(ti)(ti)生(sheng)物(wu)(wu)(wu)(wu)體(ti)(ti)(ti)內(nei)的細胞活(huo)動(dong)(dong)(dong)(dong)和基因行(xing)(xing)為。通(tong)過活(huo)體(ti)(ti)(ti)成像(xiang)(xiang)系(xi)統(tong)(tong)(tong)，可(ke)(ke)以(yi)觀測活(huo)體(ti)(ti)(ti)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)體(ti)(ti)(ti)內(nei)腫瘤的生(sheng)長(chang)及(ji)轉移，感染(ran)性疾(ji)病發(fa)展過程、特(te)定基因的表達等生(sheng)物(wu)(wu)(wu)(wu)學(xue)過程。活(huo)體(ti)(ti)(ti)成像(xiang)(xiang)系(xi)統(tong)(tong)(tong)優點(dian)是(shi)較傳統(tong)(tong)(tong)屠(tu)宰動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)相(xiang)比，該系(xi)統(tong)(tong)(tong)能夠(gou)對(dui)同一種(zhong)實(shi)驗對(dui)象在(zai)不同時(shi)間點(dian)進(jin)行(xing)(xing)記錄，跟蹤(zong)同一觀察(cha)目(mu)標（標記細胞及(ji)基因）的移動(dong)(dong)(dong)(dong)及(ji)變化，所得(de)的數(shu)據(ju)更加(jia)真(zhen)實(shi)可(ke)(ke)信。活(huo)體(ti)(ti)(ti)成像(xiang)(xiang)系(xi)統(tong)(tong)(tong)目(mu)前主要采用(yong)生(sheng)物(wu)(wu)(wu)(wu)發(fa)光與熒光兩種(zhong)技術。活(huo)體(ti)(ti)(ti)成像(xiang)(xiang)系(xi)統(tong)(tong)(tong)熒光成像(xiang)(xiang)的標記對(dui)象較為廣泛，可(ke)(ke)以(yi)是(shi)動(dong)(dong)(dong)(dong)物(wu)(wu)(wu)(wu)、細胞、微(wei)生(sheng)物(wu)(wu)(wu)(wu)、基因，也可(ke)(ke)以(yi)是(shi)抗(kang)...
2022-01-10
便捷性是全自動化學發光成像系統的一大特色
全(quan)自(zi)(zi)動(dong)(dong)化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)成像系(xi)統(tong)(tong)作(zuo)為一(yi)種分析工具的(de)(de)(de)吸(xi)引之處在(zai)于(yu)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)的(de)(de)(de)便捷性。化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)的(de)(de)(de)實質(zhi)是(shi)(shi)自(zi)(zi)身發(fa)光(guang)(guang)，這意味著化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)成像分析系(xi)統(tong)(tong)只需要提供一(yi)種可(ke)(ke)以檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)光(guang)(guang)信(xin)號和(he)記錄結(jie)果(guo)的(de)(de)(de)方(fang)法就(jiu)可(ke)(ke)以了，簡(jian)易的(de)(de)(de)化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)成像分析系(xi)統(tong)(tong)就(jiu)需要一(yi)個閉光(guang)(guang)的(de)(de)(de)樣品室和(he)光(guang)(guang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)器(qi)，簡(jian)單的(de)(de)(de)便是(shi)(shi)相(xiang)片(pian)紙或x-光(guang)(guang)片(pian)，甚至(zhi)視覺檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)器(qi)都可(ke)(ke)以。適用(yong)于(yu)化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)、多色熒(ying)光(guang)(guang)檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)與(yu)普通凝膠檢(jian)(jian)(jian)(jian)測(ce)(ce)(ce)，選用(yong)了高分辨率低照度(du)進(jin)口制冷CCD，并上(shang)乘結(jie)合大(da)光(guang)(guang)圈電動(dong)(dong)鏡頭，可(ke)(ke)捕獲到很微(wei)弱(ruo)的(de)(de)(de)熒(ying)光(guang)(guang)和(he)化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)信(xin)號。全(quan)自(zi)(zi)動(dong)(dong)化(hua)(hua)學(xue)(xue)發(fa)光(guang)(guang)成像系(xi)統(tong)(tong)的(de)(de)(de)產品特點：1、可(ke)(ke)通過(guo)儀(yi)器(qi)上(shang)的(de)(de)(de)L...
2022-01-06

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